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Transgene DNA Purification

Your transgenic construct for pronuclear microinjection should be free of vector sequences and must be pure. The Facility recommends that you separate your transgene from vector on a low-melt agarose gel using TAE buffer, and then purify your fragment using one of the following methods:

  • Qiagen QIAEX II purification kit

  • Elutip-d® columns (Note: The Facility has a supply of these columns and the buffers available for investigators.)

    • Ethanol precipitate the purified DNA, wash the pellet with 95% ethanol in order to remove the salt, and resuspend in TE. The Facility prefers to receive 5 micrograms of purified DNA.

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